Forensic Body Fluid Identification
Publication - February 2023
This article discusses specific fluorescent signatures for body fluid identification using fluorescence spectroscopy. Non-invasive, rapid, on-site detection and identification of body fluids is highly desired in forensic investigations. The use of fluorescence-based methods for body fluid identification, have so far remain relatively unexplored.
Investigation of Fluorescent Properties
In this study, the fluorescent properties of semen, serum, urine, saliva, and fingermarks over time were investigated by means of fluorescence spectroscopy, to identify specific fluorescent signatures for body fluid identification. The samples were excited at 81 different excitation wavelengths ranging from 200 to 600 nm, and for each excitation wavelength, the emission was recorded between 220 and 700 nm.
Data Analysis and Results
Subsequently, the total emitted fluorescence intensities of specific fluorescent signatures in the UV–visible range were summed, and principal component analysis was performed to cluster the body fluids. Three combinations of four principal components allowed specific clustering of the body fluids, except for fingermarks. Blind testing showed that 71.4% of the unknown samples could be correctly identified.
Fluorescent Behavior as a New Non-invasive Tool
This pilot study shows that the fluorescent behavior of ageing body fluids can be used as a new non-invasive tool for body fluid identification, which can improve the current guidelines for the detection of body fluids in forensic practice and provide the robustness of methods that rely on fluorescence.
Authors
- Nihad Achetib
- Kim Falkena
- Meghna Swayambhu
- Maurice Aalders
- Annemieke van Dam
Forensic Science research group
From DNA testing to chemical analysis: modern techniques enable examination, in minute detail, of crime-scene materials. However, success and fairness depend on correct interpretation and evaluation. The Forensic Science research group is making an important contribution via its work with the police and other parties in the criminal justice chain.